Day 5: 5/20

Follow up On Anaerobic Bacteria and Motility Test and Start of Food Test:

After a lovely game of frisbee we returned to the lab today to follow up on the experiments we started yesterday.  The first was the Thiglutinate test.  Our results were a bacteria that grew all the way through both the oxygenated and deoxygenated substance. This meant that our bacteria is facilitated anaerobic, meaning it can grow in either environments.

To follow up the the aerobic test we checked on the airtight container of or agar plates.  Unfortunately some oxygen was let into the container, and alas strip did not turn white and remained blue.  But still we could see how the bacteria grew with minimal oxygen overnight.  Our results also showed that our bacteria is anaerobic facilitated, as you can see by the growth where the inoculating tube was traced over the surface.

Today we did another trial run of the air tight container with the GasPak.

Also we checked our motility test tube.  Our bacteria was motile and the cloudiness around the inoculation lined showed the mobility of the bacteria.

Then we did a Catalase test by pouring hydrogen peroxide onto our very own plate (yay for being the odd ones out!) Because our bacteria is facilitated anaerobic there was a slight formation of bubbles meaning there was little catalase in our bacteria. This is the case because only aerobic organisms produce catalase to break down hydrogen peroxide.  Other group had more bubbles because their bacteria were anaerobe, ours had bubbles because it is facilitated anaerobic, meaning it can also grow in oxygen and does contain small amounts of catalase.

Finally we tested 10 tubes and plates for the food our bacteria eats to narrow down the type of unknown bacteria we have. Using aseptic technique we inoculated all of the tubes and plate with the following materials:
Bunsen burner
inoculating loop and needle
plate of unknown "N" bacteria




First we inoculated the starch plate, the yellow skim milk plate, DNA plate and the blue lipid plate using aseptic technique. We traced a squiggly line along on the plates.

Next we inoculated the the TSI slant tube. Sticking the inoculating needle three fourths of the way into the agar then pulled the needle out and then traced the tip along the top of the agar while pulling the needle out. We also inoculated the nutrient gelatin deep tube with three stabs with the inoculating needle.

Finally using the inoculating loop we inoculated the broth tubes of litmus milk, sucrose, lactose, maltose and glucose. Then we labeled all the tubes and plates (some many tubes and plates) and placed then on 30 degrees Celsius to grow.  This will test which nutrient the bacteria uses for growth.

                            

Results of the virus with the Unknown Bacteria:
We got out our agar plate with the virus drawn over the inoculated tube.  The bacteria had grown everywhere and the virus had killed the areas where it was traced over the surface. We put a star and our initials E.T (I mean, we are the odd ones out, right?)

Strep Test:

And last but not least we did a strep test on Theresa (gross, gag reflex :/) After taking a swab of the back of her tongue Catherine inoculated an agar plate and then put and antibacterial agent on the plate to test for streptococcus bacteria. And tomorrow we shall see if Theresa is truly and invalid!